BACTERIOLOGICAL AND PATHO-MOLECULAR STUDIES ON ARCANOBACTERIUM PYOGENES (TRUEPERELLA PYOGENES) CAUSING RESPIRATORYOR DIGESTIVESYMPTOMS IN CAMEL CALVES

This study was carried out on 83 dromedaries camel calves aged from five to eleven month old obtained from Mattroh and Salhia farms. Samples including nasal swabs, fecal samples, heparinized blood samples and samples from internal organs including lung, liver, spleen and intestine were obtained aseptically for bacterial culture, biochemical identification,
antibiogram and PCR. The bacteriological examinations revealed that Arcanobacterium pyogenes (Trueperella pyogenes) were the causative agent of the disease and isolated with percent of 14.45% from the whole samples.The organism was sensitive to Erythromycin, enrofloxacin, cefotaxime, enrofloxacin, tetracycline, Oxytetracyclin and doxycycline. The attained isolates were confirmed genotypically by successful amplification of the 16S rDNA gene by obtaining an expected band of 1403bp. Histopathological examination of camel calves revealed interstitial pneumonia, necrotizing enteritis and hepatic necrosis with splenic hyperplasia. The experimentally infected rabbits with isolated Arcanobacterium pyogenes (Trueperella pyogenes) from diseased camel calves showed similar alterations. Finally, this study concluded that A. pyogenes could be isolated from camel calves suffering from interstitial pneumonia and diarrhea. In addition, to reinforce the importance of knowing the profiles prior to start therapy,improveantimicrobial therapy approaches.