ISOLATION AND IDENTIFICATION OF S. AUREUS FROM DIFFERENT SOURCES OF BACTERIAL CONTAMINATION IN LARGE ANIMAL SLAUGHTERHOUSE

A total of 345 swap samples were collected from different sources of suspected bacterial contamination sites in cattle slaughtering hall in El-Monieb slaughter house in Giza governorate. The samples were processed for isolation and identification of coagulase positive S. aureus. The results revealed that out of 345 samples, 140 coagulase positive S. aureus samples were recorded in prevalence 40.6%. The mean count among the different sources was 4.9 ± 1.5 log10 CFU/ cm2. The sensitivity testing for 16 different antimicrobials showed that,the140 coagulase positive S.aureus samples were multidrug resistance. The electrophoretic profile of PCR for mecA gene was confirming Methicillin Resistant S. aureus isolates in 18 samples (13%). It was concluded that they are more than source of Staphylococcal contamination before, during and after slaughtering process could act as a public health risk and play a potential role in food safety issue.