CLINICOPATHOLOGICAL AND HISTOPOTHOLOGICAL STUDIES ON THE USE OF ADIPOSE DERIVED STEM CELLS IN TREATMENT OF STREPTOZOTOCIN INDUCED DIABETIC NEPHROPATHY IN RATS

Diabetic nephropathy (DN) is considered the major microvascular complication of diabetes mellitus and the main cause of chronic kidney disease. Stem cell therapy results are promising for treatment of renal tissue degeneration. Stromal vascular fraction (SVF) is easily and rapidly isolated from adipose tissue, subjected to enzymatic digestion. It is composed mainly of mesenchymal stem cells (MSCs) and other stem and regenerative cells. Forty Sprague Dawley male rats were randomly divided into four equal groups; (I) control group, (II) were diabetic untreated group, (III) and (IV) SVF single and multiple IP transplanted diabetic rats respectively. In the present study, DN was induced by single IP injection of streptozotocin (STZ) in a dose of 55 mg/ kg body weight. Adipose tissue was collected from abdominal subcutaneous region of 5 female rats, digested, centrifuged, and the SVF pellet was collected and activated using low intensity laser. Laser activated SVF was IP transplanted in group III and IV rats at the 7th day of STZ injection and injected again after two weeks in group IV. Blood and urine samples were collected three times every two weeks starting from STZ injection day. Rats were euthanized 6th weeks after STZ injection and kidney specimens were also collected. Hematological, biochemical, urine, and histopathological examinations were performed for all experimental groups. Diabetes mellitus resulted in abnormalities in the tested parameters and it were improved by transplantation of SVF. SVF multiple IP transplanted rats exhibited better improvement in all measured parameters that was confirmed by histopathological examination, thus repeated SVF transplantation is preferred for better regeneration of acute degenerated kidney