RAPID DETECTION OF FMD VIRUS SEROTYPE SAT2 IN CATTLE AND BUFFALOES BY ELISA AND REAL TIME PCR

A total of 209 vesicular fluid and tongue epithelium samples were collected from cattle and buffaloes in Sharkia (131), Ismailia (49) and Dakahlia (29) Governorates during 2018. These samples were screened for detection of FMD virus using antigen ELISA and serotyping. Thirty three samples were positive (15.7%) for SAT2 using antigen ELISA. Pan serotyping
primers were used in a real time PCR assay for detection of FMDV SAT2 virus RNA in 45 samples with a positive percentages of 21.7% and no serotype A or O were detected. Real time PCR proved to be more sensitive than antigen ELISA. FMDV is more prevalent in cattle than buffaloes with percentages of 31.5 % and 13.6 % respectively.The highest prevalence was recorded in Belbes,sharkia Governorate while the lowest rate was recorded in El-Tal keeper Ismailia Governorate with percentages of 100 and 14.2% respectively